OOC setup (no pumps included)

It is possible to couple Micronit OOC devices to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of oxygen in the culture chamber.

Have a look at this document about imaging systems.

Always check that there’s no blockage in one of the flow paths, this can be verified by checking the amount of collected liquid in a specific time frame and compare it with the expected amount for the flow rate used.

If this isn't the case, droplet formation on the end of the tubing is the problem. Each falling droplet affects the flow a bit, resulting in changes in flow rates. Resolve this by keeping the end of the tubing in the collection reservoir submerged in liquid.

Cleaning

Resealable flowcells are produced in cleanroom facilities and clean areas to prevent physical impurities. After use, cleaning might be required to remove organic residues. Please use distinguished cleaning processes for glass slides with and without a gasket.

Glass slides with gasket:

• Can be cleaned with ethanol, isopropanol and acetone. The glass slides could be either immersed in the solvents or rinsed with them. Immersion might lead to diffusion of solvents into the gasket. Therefore, it is recommended to rinse the glass slide with the gasket thoroughly with water or buffer to remove residues.
• Only wipe with lint free tissue, otherwise the sealing might be affected negatively or completely lost.
• It is advised to not wipe the gasket as this might damage it and reduce its sealing abilities.
• Physical cleaning of the surface by either heat (with a maximum temperature of 140 °C) or oxygen plasma is possible.
• Cleaning with acids or bases has not been fully tested.

Glass slides without gasket:

• Can be cleaned with organic solvents.
• Can be gently wiped with tissue.
• Physical cleaning of the surface by either heat up to 400 °C or oxygen plasma treatment is possible.
• Alkaline solution can be used to clean the surface, e.g. 1 M sodium hydroxide in water.
• Concentrated sulfuric acids could dissolve organic material, but are extremely corrosive, therefore it is not advised to use those. Hydrochloric acid could be used for cleaning, as well as bleach.

Sterilization

Resealable flowcells are not sterilized when delivered. Please use distinguished sterilization processes for glass slides with and without a gasket.

Glass slides with gasket:

• Can be sterilized with ethanol.
• Dry heat of up to 140 °C can be applied.
• Can be autoclaved.

Glass slides without gasket:

• Can be sterilized with ethanol.
• Can be autoclaved.

The oxygen permeability data from the exact material grade used is not available, however the  data  from a different grade of the same material family, can be found below. 

Nuts and ferrules

Elveflow uses 1/4-28 screw in connections on there reservoirs and sensors. To interface you will need Flangeless nuts 1/4-28 for 1/16" tubing, including compatible ferrules. Those nuts and ferrules are included in Elveflows "Kit Fittings Starter Pack Push-in".
This item wil look like this:

In addition you will need our connection kit. Our Connection kit includes nuts or ferrules specificilly designed for our Fluidic Connect Pro, Fluidic Connect 4515 or side connect holders.

Tubing included in "Kit Fittings Starter Pack Push-in"

The 1/16"OD PTFE tubing included in the kit has a very large ID (1/32" ~0.79mm) and is from a relatively soft material, this is not so suitable for bot our Fluidic Connect Pro and Fluidic Connect 4515 holders as there's very limited wall thickness. We recommend alternative tubing, like ETFE with a maximum of 0.5mm ID. This tubing is included in our connection kits.

Use of 1/32" tubing
1/32" tubing can be in combination with our Fluidic Connect Pro holder also be used, but need alternative ferrules. The ElveFlow "Kit Fittings Starter Pack Push-in" doesn't include sleeves for use of 1/32" OD tubing. They are included in our "Syringe to tube connection kit"
Our Sideconnect our Fluidic Connect 4515 holders doens't support 1/32" tubing.

Lineup

We recommend the dafault Fluigent kits (FLOW EZ™ SUPPLY KIT, FLUIWELL KIT or P-CAP KIT)
The P-cab or FLUIWELL KIT already contains 0.5m 1/16" OD FEP tubing with a ID of 500µm, when you use only limited pressures, you can try this tubing first and only order:

• For Fluidic Connect Pro our propriatary ferrules. 
• For Fluidic Connect 4515 our propriatary flatbottom nuts and ferrules. 
• For Sideconnect our propriatary ferrules. 

For optimal performance we recommend our complete connection kit, the FEP tubing might be a bit soft limiting the pressure rating. 

MFCS

We recommend the dafault Fluigent kits (MFCS™-EZ HIGH/LOW PRESSURE KIT, FLUIWELL KIT or P-CAP KIT ) 

The P-cab or FLUIWELL KIT already contains 0.5m or 1m 1/16" OD FEP tubing with a ID of 500µm, when you use only limited pressures, you can try this tubing first and only order:

• For Fluidic Connect Pro our propriatary ferrules. 
• For Fluidic Connect 4515 our propriatary flatbottom nuts and ferrules. 
• For Sideconnect our propriatary ferrules. 

For optimal performance we recommend our complete connection kit, the FEP tubing might be a bit soft limiting the pressure rating.