Basic microfluidic setup - resealable (no pumps included)

From €2,609.00

To €3,335.00

In stock

€2,609.00

This setup includes a set of components to create a resealable flow cell setup. Apart from the components in this bundle, you will need your required (bio)content, a pump to create flow and equipment for the observation of your experiment.

1 x Fluidic Connect Pro chip holder - topconnect - for resealable flow cells   + €1,950.00
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1 x Fluidic Connect Pro ETFE connection kit   + €159.00
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A product bundle to create a setup for an easy start with our resealable flow cell technology.

Resealable flow cells are an excellent choice when biological content needs to be added that can't be flushed inside. By use of a resealable flow cell it is easy to create a biological sensor. Just spot or dispence your biological content on the provided glass slide, place inside the special holder, add the top layer and close the hinge.

The chips consists of two layers, a top and bottom layer:

  • The bottom layer is a plain glass slide, with a thickness of 700µm.
  • The top layer is a 1100µm thick glass layer with an elastomer attached to it. The elastomer will define the flow path and will seal the top layer.

The optional syringe to pump connection kit is recommended in combination with syringe pumps, as it contains a connector that will connect between a Luer-Lock syringe and the tubing. 

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Customer Questions
How do I clean my resealable flow cell's / OOC top and bottom layers?

Cleaning

Resealable flowcells are produced in cleanroom facilities and clean areas to prevent physical impurities. After use, cleaning might be required to remove organic residues. Please use distinguished cleaning processes for glass slides with and without a gasket.

Glass slides with gasket:

  • Can be cleaned with ethanol, isopropanol and acetone. The glass slides could be either immersed in the solvents or rinsed with them. Immersion might lead to diffusion of solvents into the gasket. Therefore, it is recommended to rinse the glass slide with the gasket thoroughly with water or buffer to remove residues.
  • Only wipe with lint free tissue, otherwise the sealing might be affected negatively or completely lost.
  • It is advised to not wipe the gasket as this might damage it and reduce its sealing abilities.
  • Physical cleaning of the surface by either heat (with a maximum temperature of 140 °C) or oxygen plasma is possible.
  • Cleaning with acids or bases has not been fully tested.

Glass slides without gasket:

  • Can be cleaned with organic solvents.
  • Can be gently wiped with tissue.
  • Physical cleaning of the surface by either heat up to 400 °C or oxygen plasma treatment is possible.
  • Alkaline solution can be used to clean the surface, e.g. 1 M sodium hydroxide in water.
  • Concentrated sulfuric acids could dissolve organic material, but are extremely corrosive, therefore it is not advised to use those. Hydrochloric acid could be used for cleaning, as well as bleach.

How do I sterilize my resealable flow cell's / OOC top and bottom layers?

Sterilization

Resealable flowcells are not sterilized when delivered. Please use distinguished sterilization processes for glass slides with and without a gasket.

Glass slides with gasket:

  • Can be sterilized with ethanol.
  • Dry heat of up to 140 °C can be applied.
  • Can be autoclaved.

Glass slides without gasket:

  • Can be sterilized with ethanol.
  • Can be autoclaved.

I have a Elveflow OB1 pressure based pump what connectors do I need?

Nuts and ferrules

Elveflow uses 1/4-28 screw in connections on there reservoirs and sensors. To interface you will need Flangeless nuts 1/4-28 for 1/16" tubing, including compatible ferrules. Those nuts and ferrules are included in Elveflows "Kit Fittings Starter Pack Push-in".
This item wil look like this:

In addition you will need our connection kit. Our Connection kit includes nuts or ferrules specificilly designed for our Fluidic Connect Pro, Fluidic Connect 4515 or side connect holders.

Tubing included in "Kit Fittings Starter Pack Push-in"

The 1/16"OD PTFE tubing included in the kit has a very large ID (1/32" ~0.79mm) and is from a relatively soft material, this is not so suitable for bot our Fluidic Connect Pro and Fluidic Connect 4515 holders as there's very limited wall thickness. We recommend alternative tubing, like ETFE with a maximum of 0.5mm ID. This tubing is included in our connection kits.

Use of 1/32" tubing
1/32" tubing can be in combination with our Fluidic Connect Pro holder also be used, but need alternative ferrules. The ElveFlow "Kit Fittings Starter Pack Push-in" doesn't include sleeves for use of 1/32" OD tubing. They are included in our "Syringe to tube connection kit"
Our Sideconnect our Fluidic Connect 4515 holders doens't support 1/32" tubing.

I have a Fluigent pressure based pump, what connectors do I need?

Lineup

We recommend the dafault Fluigent kits (FLOW EZ™ SUPPLY KIT, FLUIWELL KIT or P-CAP KIT)
The P-cab or FLUIWELL KIT already contains 0.5m 1/16" OD FEP tubing with a ID of 500µm, when you use only limited pressures, you can try this tubing first and only order:

For optimal performance we recommend our complete connection kit, the FEP tubing might be a bit soft limiting the pressure rating. 

MFCS

We recommend the dafault Fluigent kits (MFCS™-EZ HIGH/LOW PRESSURE KIT, FLUIWELL KIT or P-CAP KIT ) 

The P-cab or FLUIWELL KIT already contains 0.5m or 1m 1/16" OD FEP tubing with a ID of 500µm, when you use only limited pressures, you can try this tubing first and only order:

For optimal performance we recommend our complete connection kit, the FEP tubing might be a bit soft limiting the pressure rating. 

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