Focused flow droplet generator - topconnect
per pack of 3
Pack of 3 focused flow droplet generators for use in combination with our Fluidic Connect Pro chip holder. The chips are available with a hydrophobic coated or an uncoated (hydrophilic) channel surface.
Indicated reference droplet sizes are based on a reference experiment with Silicone oil 5cSt and DI water combined with 2% v/v Tween 20 or Tween 80.
Pack of 3 single nozzle droplet generators with a nozzle etched on both sides, giving the following advantages:
- Optimized nozzle geometry
- Symmetric channels and nozzles for uniform droplet formation
- Droplet production stable over a broader size and frequency range
- Smaller droplet sizes available
This microfluidic droplet generator is an excellent tool for generating highly reproducible microsized droplets with much higher precision and repeatability compared to conventional methods.
By tuning the relative viscosities, surface tension, and velocities between the dispersed and the continuous phase, droplet size and frequency can be altered. Oil-in-Water (O/W) droplets can be generated directly using the glass chips. Also, these droplet generators are suitable for coating in order to form Water-in-Oil droplets (W/O).
- Droplet size can roughly be tuned from the size of the nozzle to be doubled
- Suitable for foam, digital PCR, single cell analysis, emulsions etc.
- Made from high quality glass, borosilicate, suitable for most biological and chemical applications
Cell, DNA, bead encapsulation for
- drug discovery
- drug studies
- molecular biological studies
- immunology studies
- evolutional studies
- enzyme catalysis studies
Food, paints, foams
- Bubble formation
- Mineral Oil Emulsion Production
- Particle production - PLGA, PEGDA, gelatine, alginate, polystyrene, agaros
- Drug delivery - creams, aerosols
- Droplet based micromixing
- Droplet based microreactions
|Unit of measurement
|pack of 3
|Supply format & dimensions
Supplied in a black polymer cardtridge with an external dimension of 75 x 25mm.
|Depending on selection
|Number of inlets
|Number of outlets
|11000935 / 11001341 / DGFF.10 - Drawing
|Drawing for 10µm Topconnect Focused Flow Droplet Generator. Items 11001341 and 11000935.
|11000877 / 11006011 / DGFF.50 - Drawing
|Drawing for 50µm Topconnect Focused Flow Droplet Generator. Items 11006011 and 11000877.
|11000970 / 11000580 / DGFF.75 - Drawing
|Drawing for 75µm Topconnect Focused Flow Droplet Generator. Items 11000970 and 11000580.
|Surface wetting properties
|Flow rate instructions
|How to prevent clogging
|Using the right surfactants
|Droplet Generator Guide
To remove particulate matter from your chip, a water bath with ultrasonic agitation can be used, preferably while flushing a watery solution through the channels.
Glass microchips can be heated (e.g. 400°C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. Make sure you only heat the glass chip and not the plastic parts around it.
Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions. Always keep in mind that you are working with extremely corrosive material.
Please note that chips that were coated by Micronit have different guidelines for cleaning!
The objective working distance is a critical parameter for selection of an objective. Most default objectives are indented for a #1.5 cover slip which is only 170µm thick, where the thickness below channel is mostly in the range of 400-900µm.
Those objectives with longer working distance are often called non-coverglass objectives.
Where possible we would recommend to work dry. In most cases it should be possible to use the channel edge or other well defined point as reference for manual size corrections, this would reduce the need for corrections by the objective.
Large roughness for structures in glass chips is typical observed for structures manufactured by use of laser assisted manufacturing techniques or abrasion-based techniques like powder blasting. Almost all catalogue products from Micronit are manufactured using wet etching to create full transparent channels without substantial roughness.