Focused flow droplet generator - topconnect
Pack of 3 focused flow droplet generators for use in combination with our Fluidic Connect Pro chip holder. The chips are available with a hydrophobic coated or an uncoated (hydrophilic) channel surface.
Indicated reference droplet sizes are based on a reference experiment with Silicone oil 5cSt and DI water combined with 2% v/v Tween 20 or Tween 80.
Pack of 3 single nozzle droplet generators with a nozzle etched on both sides, giving the following advantages:
- Optimized nozzle geometry
- Symmetric channels and nozzles for uniform droplet formation
- Droplet production stable over a broader size and frequency range
- Smaller droplet sizes available
This microfluidic droplet generator is an excellent tool for generating highly reproducible microsized droplets with much higher precision and repeatability compared to conventional methods.
By tuning the relative viscosities, surface tension, and velocities between the dispersed and the continuous phase, droplet size and frequency can be altered. Oil-in-Water (O/W) droplets can be generated directly using the glass chips. Also, these droplet generators are suitable for coating in order to form Water-in-Oil droplets (W/O).
- Droplet size can roughly be tuned from the size of the nozzle to be doubled
- Suitable for foam, digital PCR, single cell analysis, emulsions etc.
- Made from high quality glass, borosilicate, suitable for most biological and chemical applications
Cell, DNA, bead encapsulation for
- drug discovery
- drug studies
- molecular biological studies
- immunology studies
- evolutional studies
- enzyme catalysis studies
Food, paints, foams
- Bubble formation
- Mineral Oil Emulsion Production
- Particle production - PLGA, PEGDA, gelatine, alginate, polystyrene, agaros
- Drug delivery - creams, aerosols
- Droplet based micromixing
- Droplet based microreactions
|Unit of measurement||3-Pack|
|Use Custom Stock Message||No|
|Custom Stock Is Available (Icon)||No|
|Chip material||Borosilicate glass|
|Supply format & dimensions|
Supplied in a black polymer cardtridge with an external dimension of 75 x 25mm.
|Channel location||Depending on selection|
|Number of inlets||2|
|Number of outlets||1|
|DGFF.10 - Drawing||Drawing for 10µm Topconnect Focused Flow Droplet Generator. Items 01341 and 00935.||159.2 KB||Download|
|DGFF.50 - Drawing||Drawing for 50µm Topconnect Focused Flow Droplet Generator. Items 01366 and 00877.||179.6 KB||Download|
|DGFF.75 - Drawing||Drawing for 75µm Topconnect Focused Flow Droplet Generator. Items 00970 and 00580.||193.6 KB||Download|
|Surface wetting properties||220.3 KB||Download|
|Flow rate instructions||303.6 KB||Download|
|Using the right surfactants||168.8 KB||Download|
|How to prevent clogging||153 KB||Download|
|Droplet Generator Guide||617 KB||Download|
One simple but very effective way to clean a microchip is to flush an alkaline solution through the channels. A solution of 1 M sodium hydroxide in water works well, but a lower concentration might also be sufficient. If traces of the cleaning solution...
We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles.
To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers: https://www.fluigent.com/
This depends on many things. For example on the type of fluid that you are using. Check our flowrate instructions to find out how to start.
Decrease your flowrate. Check our flowrate instructions for a more acurate explanation.
Use our surfactant guide for advice on surfactants.
Have a look at our clogging prevention guide.
Have a look at our article about surface wetting properties.
Amoyav, Benzion, and Ofra Benny "Controlled and tunable polymer particles’ production using a single microfluidic device." Applied Nanoscience (2018): 1-10. Abstract Microfluidics technology offers a new platform to control liquids under flow in small...
Lucio, Adam A., et al. "Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids." Scientific reports 7.1 (2017): 12022. Abstract Multicellular spheroids serve as an excellent platform to study tissue behavior...