Classic focused flow droplet generator

SKU
FFDROP_CLASIC
Availability:
In stock
As low as €490.00

per pack of 3

Classic droplet generator. The channels have half semi-oval shape.

 

Droplet generation is a important technique for emulsification and single cell encapsultation. Also in Pharmaceutical applications, droplet generation can play an important role in drug delivery, when active ingredients are mixed with biological polymers. 

Micornit offers currently two versions of single junction focused flow droplet generators, we offer a "classic" and a new generation version. In most case the new generation droplet generators are the prefered choice. However the classic version helps to determine if a half semi-oval channel shape can be sufficient as this is relatively cost effective design. Because it's was one of the first commercial availble droplet generatore chips it's also referenced in a large amount of publications.

  Half semi-oval channel shape  ("classic")                                                      Full semi-oval channel shape ("new generation")

The "classic" droplet generator is available in the following configurations:

SKU Nozzle height
[µm]
Hydrophobic Coating

Channel height
[µm]

Channel width
[µm]

02889 10 No 20 100
02892 10 Yes 20 100
02890 50 No 100 500
02891 50 Yes 100 500

More details are displayed when the desired configuration is selected.

More Information
Unit of measurementpack of 3
Interface typeTopconnect
Chip materialBorosilicate glass
Customer Questions
How do I clean my chips?
One simple but very effective way to clean a microchip is to flush an alkaline solution through the channels. A solution of 1 M sodium hydroxide in water works well, but a lower concentration might also be sufficient. If traces of the cleaning solution remain inside the chip after cleaning, rinse with water or ammonia. Note that caustic solutions can cause damage to e.g. the polyimide coating of fused silica capillaries. Further, plastic parts should not be exposed to alkaline solutions. To remove particulate matter from your chip, a water bath with ultrasonic agitation can be used, preferably while flushing a watery solution through the channels using a Fluidic connection kit. Glass microchips can be heated (e.g. >400° C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. Make sure you only heat the glass chip and not the plastic parts around it. Concentrated sulfuric acid works well ...
I need a pumping system for my setup. Which one do you recommend?
We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles. To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers: https://www.fluigent.com/  
Which flowrates should I use?
This depends on many things. For example on the type of fluid that you are using. Check our flowrate instructions to find out how to start.
I only see streaks of fluids but no droplets. How do I get the droplets?
Decrease your flowrate. Check our flowrate instructions for a more acurate explanation.
Which surfactants should I use?
Use our surfactant guide for advice on surfactants.
Should I use coated or uncoated droplet generators?
Have a look at our article about surface wetting properties.
Publication: Controlled and tunable polymer particles' production using a single microfluidic device
Amoyav, Benzion, and Ofra Benny "Controlled and tunable polymer particles’ production using a single microfluidic device." Applied Nanoscience (2018): 1-10. Abstract Microfluidics technology offers a new platform to control liquids under flow in small volumes. The advantage of using smallscale reactions for droplet generation along with the capacity to control the preparation parameters, making microfluidic chips an attractive technology for optimizing encapsulation formulations. However, one of the drawbacks in this methodology is the ability to obtain a wide range of droplet sizes, from sub-micron to microns using a single chip design. In fact, typically, droplet chips are used for micron-dimension particles, while nanoparticles’ synthesis requires complex chips design (i.e., microreactors and staggered herringbone micromixer). Here, we introduce the development of a highly...
Publication: Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids
Lucio, Adam A., et al. "Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids." Scientific reports 7.1 (2017): 12022. Abstract Multicellular spheroids serve as an excellent platform to study tissue behavior and tumor growth in a controlled, three-dimensional (3D) environment. While molecular and cellular studies have long used this platform to study cell behavior in 3D, only recently have studies using multicellular spheroids shown an important role for the mechanics of the microenvironment in a wide range of cellular processes, including during tumor progression. Despite the well-established relevance of mechanical cues to cell behavior and the numerous studies on mechanics using 2D cell culture systems, the spatial and temporal variations in endogenous cellular forces within growing multicellular aggregates remain unknown. Using cell-sized oil ...
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