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Pack of 3 single nozzle droplet generators with nozzle etched on both sides, giving the following advantages:
This microfluidic droplet generator is an excellent tool for generating highly reproducible microsized droplets with much higher precision and repeatability compared to conventional methods.
By tuning the relative viscosities, surface tension, and velocities between the dispersed and the continuous phase, droplet size and frequency can be altered. Two versions of this chip are available, A chip for oil-in-water (O/W) droplets and a chip for water-in-oil droplets. The chips for oil-in-water have an untreated glass surface that is hydrophilic and the water-in-oil chips have an extra coating making them hydrophobic.
This droplet generator has the same functionality as our top-connected droplet generator with the same nozzle size. This version has the following advantages compared to the top connect version:
A disadvantage of this holder is that it is slightly less convenient to connect and disconnect chips into the holder. Therefore, if the mentioned advantages are not relevant to you, choose the top-connected droplet generators
Cell, DNA, bead encapsulation for
Food, paints, foams
|Product reference for order|
Please use the following product references for an order:
|Number of chips per pack||3|
|Distance between channel and top surface||696 µm|
|Distance between channel and bottom surface||696 µm|
|Channel location||Top and bottom|
|Total chip thickness||1400 µm|
|Chip size||15 mm x 15 mm|
|Channel height||8 µm|
|Internal Volume||0.09 µl|
|Nozzle height||75 µm|
|Number of Inlets||3 (2 inlet are used for continuous phase)|
|Number of outlets||1|
|Optical properties||Optical clear view from all sides|
|Coating||Depend on selection|
|Material chip||Borosilicate glass|
One simple but very effective way to clean a microchip is to flush an alkaline solution through the channels. A solution of 1 M sodium hydroxide in water works well but a lower concentration might also be sufficient. If traces of the cleaning solution remaining inside the chip after cleaning and rinsing with water pose a problem then ammonia can be used instead. Note that these solutions are caustic and can cause damage to e.g. the polyimide coating of fused silica capillaries. Also plastic parts should not be exposed to very alkaline solutions.
In order to aid in the removal of particulate matter, a water bath with ultrasonic agitation can be used, preferably while flushing a watery solution through the channels using a Fluidic connect kit.
Glass microchips can be heated (e.g. >400° C) causing any organic material adsorbed on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. Make sure you only heat the glass chip and not the plastic parts around it.
Concentrated sulphuric acid works well to dissolve organic material such as fibres which are difficult to remove with alkaline solutions, but because of the extremely corrosive nature of the material a cleaning procedure is not so easily implemented.
Please note that chips that were coated by Micronit have different guidelines for cleaning.
Chips that have Micronit’s standard hydrophobic coating can be cleaned with most organic solvents. IPA, acetone, ethanol and water should all be safe to use without damaging the coating. Do not clean the chips with any very acidic or alkaline chemical. Also, flush the chip with your cleaning material but don’t store the chip for many days with an organic solvent inside. Coated chips can be stored filled with water or air.
This depends on many things, for example, which fluids you use. Check our flowrate instructions to find out how to start.
Decrease your flowrate. Check our flowrate instructions for a more acurate explanation.
Have a look at our surfactant guide.
Have a look at our clogging prevention guide
Is your setup clogged with dirt or fully blocked?
Chips can get clogged, for example when they’ve been used before and have not been cleaned the right way. If your chip is clogged with dirt, check our document on the prevention of clogging and/or read the answer to question number one on this page (regarding the cleaning of chips). Clogged chips often do let some flow through the chip, which makes it easier to identify the issue as clogging. If there is no flow at all it’s more likely that the flow path is not connected correctly.
Pinpoint the issue
We recommend walking through the full track of the fluid flows: check if your syringe or pump is not blocked, if your tubing is actually hollow and if your filters let fluids pass through. Then check if your chip has inlet and outlet holes and if these are positioned so that the fluids can go through the chip. The easiest way to do this is to disassemble the setup and check if fluids come out of the pump, then connect the tubing and check if fluids can come through. Keep on adding parts and checking if the fluids go through. This way you can pinpoint the problem.
If the problem remains
If the problem cannot be solved after following the above-mentioned steps, then take some pictures or make a short movie of your setup and send this, together with the description or name of your chip, to Micronit. We will gladly assist you in identifying the issue.
Have a look at our document about surface wetting properties.