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These thin bottom flowcells are ideal for experiments such as cell, particle or DNA imaging. The thin 145 micrometer bottom plate makes it suitable for confocal microscopy.
Pack of 3 chips.
For connecting all three channels at the same time, you need 6 ferrules. The ferrules are delivered in sets of 5. Make sure you buy two sets if you want to connect three channels at the same time.
Here is a paper using this chip to collect individual chromosomes:
Yusuf, Mohammed, et al. "Development of a robotic system interfaced to a microfl uidic device to isolate a single chromosome." Chromosome Science 18.3-4 (2015): 73-77.
Here is a paper using the flow cell to study diffusion and interaction of emulsion droplets
Griffiths, Marjorie R., et al. "Measuring the interaction between a pair of emulsion droplets using dual-trap optical tweezers." RSC Advances 6.18 (2016): 14538-14546.
|Number of chips per pack||3|
|Distance between channel and top surface||1050 µm|
|Distance between channel and bottom surface||145 µm|
|Total chip thickness||1245 µm|
|Chip size||45 mm x 15 mm|
|Channel width||500 µm, 1500 µm and 1000 µm|
|Channel height||50 µm|
|Internal Volume||1.0 µl, 2.8 µl and 1.9 µl|
|Number of Inlets||3|
|Number of outlets||3|
|Inlet/outlet hole sizes on top of the chip||1.70 mm|
|Inlet/outlet holes size at channel||0.60 mm|
|Optical properties||Optical clear view from all sides|
|Supplied in Fluidic slide?||Yes|
|Material chip||Borosilicate glass|
|Material black cartridge||Polypropylene|
One simple but very effective way to clean a microchip is to flush an alkaline solution through the channels. A solution of 1 M sodium hydroxide in water works well but a lower concentration might also be sufficient. If traces of the cleaning solution remaining inside the chip after cleaning and rinsing with water pose a problem then ammonia can be used instead. Note that these solutions are caustic and can cause damage to e.g. the polyimide coating of fused silica capillaries. Also plastic parts should not be exposed to very alkaline solutions.
In order to aid in the removal of particulate matter, a water bath with ultrasonic agitation can be used, preferably while flushing a watery solution through the channels using a Fluidic connect kit.
Glass microchips can be heated (e.g. >400° C) causing any organic material adsorbed on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. Make sure you only heat the glass chip and not the plastic parts around it.
Concentrated sulphuric acid works well to dissolve organic material such as fibres which are difficult to remove with alkaline solutions, but because of the extremely corrosive nature of the material a cleaning procedure is not so easily implemented.
Please note that chips that were coated by Micronit have different guidelines for cleaning.
Is your setup clogged with dirt or fully blocked?
Chips can get clogged, for example when they’ve been used before and have not been cleaned the right way. If your chip is clogged with dirt, check our document on the prevention of clogging and/or read the answer to question number one on this page (regarding the cleaning of chips). Clogged chips often do let some flow through the chip, which makes it easier to identify the issue as clogging. If there is no flow at all it’s more likely that the flow path is not connected correctly.
Pinpoint the issue
We recommend walking through the full track of the fluid flows: check if your syringe or pump is not blocked, if your tubing is actually hollow and if your filters let fluids pass through. Then check if your chip has inlet and outlet holes and if these are positioned so that the fluids can go through the chip. The easiest way to do this is to disassemble the setup and check if fluids come out of the pump, then connect the tubing and check if fluids can come through. Keep on adding parts and checking if the fluids go through. This way you can pinpoint the problem.
If the problem remains
If the problem cannot be solved after following the above-mentioned steps, then take some pictures or make a short movie of your setup and send this, together with the description or name of your chip, to Micronit. We will gladly assist you in identifying the issue.