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12-Pack OOC Membrane Layers

Availability: Manufactured to order, estimate lead time 3-4 weeks.

Pack of 12 middle layers only. To be placed in between the top and bottom OOC layers (product 00739)


The layer containing the cell culture membrane is placed in between two resealable glass slides to form two separate flow chambers. This allows the flow of two different fluids, either liquids or gases, on either side of the membrane. This dynamic microfluidic flow approach enables new and innovative ways to culture cells and tissues while offering precise and continuous control during the complete culturing process. The membrane layer forms together with a top and bottom layer (product 00739) an organ-on-a-chip resealable flow cell.

In this paper the 0.45µm membrane was used:
Navarro-Tableros, Victor, et al. "Islet-Like Structures Generated In Vitro from Adult Human Liver Stem Cells Revert Hyperglycemia in Diabetic SCID Mice." Stem Cell Reviews and Reports (2018): 1-19.

In this paper the 0.45µm membrane was used:
Yonathan Gomez, et al. "A Versatile Model of Microfluidic Perifusion System for the Evaluation of C-Peptide Secretion Profiles: Comparison Between Human Pancreatic Islets and HLSC-Derived Islet-Like Structures", Biomedicines, 2020 - mdpi.com

In this paper the 3µm membrane was used:
Li, Xiang, et al. "A glass-based, continuously zonated and vascularized human liver acinus microphysiological system (vLAMPS) designed for experimental modeling of diseases and ADME/TOX." Lab on a Chip 18.17 (2018): 2614-2631.

Product Codeooc_membrane
Product reference for order

Please use the following product references for an order:
- 0.45µm pore size:  00738
- 3µm pore size:       01206
- 8µm pore size:       01060

Number of chips per pack12 (middle layers only)
Layer Thickness Carrier0.4 mm
Material Carrier LayerBorosilicate glass
Membrane MaterialPET
Thickness Membrane0.45µm pore size: 12µm thickness; 3µm µm pore size: 9µm; 8µm µm pore size: 16µm
Membrane Surface~1 cm²
Membrane Pore Density0.45µm pore size: 2.00 E+6 (cm2); 3µm pore size: 8.00E+05; 8µm pore size: 6.00 E+4 (cm2)
Membrane AppearanceTransparent
Membrane Surface TreatmentCell culture treated

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How do I clean and sterilize my resealable flow cells?

Is it possible to monitor oxygen levels?

It is possible to couple Micronit OOC device to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of the oxygen in the culture chamber.

Is it possible to control the oxygen concentration?

Micronit's OOC device is made of three glass layers. Glass has a very low gas permeability, making the system suitable for controlling gas concentrations. In case you use a syringe pump, the medium must be pre-conditioned to the right gas concentration. In case you use pneumatic pressurization systems, like Fluigent systems, specific gas mixtures can be used to condition the liquids during perfusion.

Is my OOC system compatible with my imaging systems?

The flow is periodically switching between top and bottom flow path.

When the bellow characteristics are overserved: 
- Flow is periodically switching between the collection reservoir for the top and bottom flow path. 
- Displacement of the membrane.
- Both flow paths are functioning correct, the reservoirs contains an amount of liquid that is expected based on the flow rates.

This is caused by droplet formation on the collection tubing. Each drop affects the flow a bit, resulting in changes in flow rates. This can be resolved by keeping the end of the tubing in the collection reservoir submerged in liquid.

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