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4-Pack Top and Bottom OOC Layers

Availability: In stock

Two resealable glass slides that form the top and bottom layers for the organ-on-a-chip devices.
Two resealable glass slides that form the top and bottom layers for the organ-on-a-chip devices. When the layer containing the membrane (product code 00738) is placed in between these top and bottom layers two separate flow chambers are formed.For this product a special OOC holder/ or OOC insert set is required.
Product Code00739
Number of chips per pack4 Top layers, 4 bottom layers
Distance between channel and top surfaceThickness top layer 1.1 mm
Distance between channel and bottom surfaceThickness bottom layer 0.7 mm
Total chip thickness2.6 mm (including middle layer)
Chip size45 mm x15 mm
Channel width11 mm, 2 channel, 1x on top of membrane, 1x on bottom of membrane
Channel height~200 µm (per channel)
Internal Volume-
Number of Inlets2 (1 inlet for flow channel on top membrane, 1 inlet for flow on bottom of membrane )
Number of outlets2 (1 outlet for flow channel on top membrane, 1 outlet for flow on bottom of membrane )
Inlet/outlet hole sizes on top of the chip0.75 mm
Inlet/outlet holes size at channel1.70 mm
Optical propertiesOptical clear view from all sides
Supplied in Fluidic slide?No
Material chipBorosilicate glass
Material black cartridge-

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How do I clean and sterilize my resealable flow cells?

How do I clean the Top and Bottom OOC layers?

To clean and sterilize the Top and Bottom OOC layers you can follow the Cleaning and sterilization procedure for the resealable flow cells.

Is it possible to monitor oxygen levels?

It is possible to couple Micronit OOC device to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of the oxygen in the culture chamber.

Is it possible to control the oxygen concentration?

Micronit's OOC device is made of three glass layers. Glass has a very low gas permeability, making the system suitable for controlling gas concentrations. In case you use a syringe pump, the medium must be pre-conditioned to the right gas concentration. In case you use pneumatic pressurization systems, like Fluigent systems, specific gas mixtures can be used to condition the liquids during perfusion.

Is my OOC system compatible with my imaging systems?

The flow is periodically switching between top and bottom flow path.

When the bellow characteristics are overserved: 
- Flow is periodically switching between the collection reservoir for the top and bottom flow path. 
- Displacement of the membrane.
- Both flow paths are functioning correct, the reservoirs contains an amount of liquid that is expected based on the flow rates.

This is caused by droplet formation on the collection tubing. Each drop affects the flow a bit, resulting in changes in flow rates. This can be resolved by keeping the end of the tubing in the collection reservoir submerged in liquid.

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