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|Number of chips per pack||4 Top layers, 4 bottom layers|
|Distance between channel and top surface||Thickness top layer 1.1 mm|
|Distance between channel and bottom surface||Thickness bottom layer 0.7 mm|
|Total chip thickness||2.6 mm (including middle layer)|
|Chip size||45 mm x15 mm|
|Channel width||11 mm, 2 channel, 1x on top of membrane, 1x on bottom of membrane|
|Channel height||~200 µm (per channel)|
|Number of Inlets||2 (1 inlet for flow channel on top membrane, 1 inlet for flow on bottom of membrane )|
|Number of outlets||2 (1 outlet for flow channel on top membrane, 1 outlet for flow on bottom of membrane )|
|Inlet/outlet hole sizes on top of the chip||0.75 mm|
|Inlet/outlet holes size at channel||1.70 mm|
|Optical properties||Optical clear view from all sides|
|Supplied in Fluidic slide?||No|
|Material chip||Borosilicate glass|
|Material black cartridge||-|
Check and follow the Cleaning and sterilization procedure for resealable flow cells.
To clean and sterilize the Top and Bottom OOC layers you can follow the Cleaning and sterilization procedure for the resealable flow cells.
It is possible to couple Micronit OOC device to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of the oxygen in the culture chamber.
Micronit's OOC device is made of three glass layers. Glass has a very low gas permeability, making the system suitable for controlling gas concentrations. In case you use a syringe pump, the medium must be pre-conditioned to the right gas concentration. In case you use pneumatic pressurization systems, like Fluigent systems, specific gas mixtures can be used to condition the liquids during perfusion.
Have a look at this document about imaging systems.
When the bellow characteristics are overserved:
- Flow is periodically switching between the collection reservoir for the top and bottom flow path.
- Displacement of the membrane.
- Both flow paths are functioning correct, the reservoirs contains an amount of liquid that is expected based on the flow rates.
This is caused by droplet formation on the collection tubing. Each drop affects the flow a bit, resulting in changes in flow rates. This can be resolved by keeping the end of the tubing in the collection reservoir submerged in liquid.