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The ‘Organ-on-a-Chip Platform’ is a great place to start for researchers aiming to develop their own organ-on-a-chip [OOC] applications. This platform provides a double chamber flow cell and its interfacing. All you need to add is pumps and materials such as cells and fluids. what remains for you is doing what you are good at; biology!
The flow cell is resealable and can be opened. It consists of two chambers where flow can be individually controlled. The membrane separates the two chambers.
Micronit does not do biological research on these components, but our customers do! Have a look at the three papers below in which our items were used. These papers show you more about the devices, they show you can do complex biology on them and they proof you can publish using them.
Li, Xiang, et al. "A glass-based, continuously zonated and vascularized human liver acinus microphysiological system (vLAMPS) designed for experimental modeling of diseases and ADME/TOX." Lab on a Chip 18.17 (2018): 2614-2631.
Yonathan Gomez, et al. "A Versatile Model of Microfluidic Perifusion System for the Evaluation of C-Peptide Secretion Profiles: Comparison Between Human Pancreatic Islets and HLSC-Derived Islet-Like Structures", Biomedicines, 2020 - mdpi.com
|Product Code||Organ-on-a-Chip Platform|
|Dimensions||Chipholder: 128 mm x 85,4 mm x 20 mm|
|Sealing mechanism||Load ‘n Seal|
|Maximum operation temperature||80°C|
|Max. operating pressure||0.4 bar (OOC version)|
|Sealing material||Perlast (FFKM)|
|Contents||Connection Kit: ETFE tubing and 5 FFKM ferrules|
|Length||5 m tubing|
|Outer Diameter (OD)||1/16 Inch (~1.6 mm)|
|Inner Diameter (ID)||250 µm|
|Number of chips per pack||4 top and bottom layers, 12 membrane middle layers|
|Distance between channel and top surface||Thickness top layer 1.1 mm|
|Distance between channel and bottom surface||Thickness bottom layer 0.7 mm|
|Total chip thickness||2.6 mm (including middle layer)|
|Chip size||45 mm x15 mm|
|Channel width||11 mm, 2 channel, 1x on top of membrane, 1x on bottom of membrane|
|Channel height||~200 µm (per channel)|
|Number of Inlets||2 (1 inlet for flow channel on top membrane, 1 inlet for flow on bottom of membrane )|
|Number of outlets||2 (1 outlet for flow channel on top membrane, 1 outlet for flow on bottom of membrane )|
|Inlet/outlet hole sizes on top of the chip||1.7 mm|
|Inlet/outlet holes size at channel||0.75 mm|
|Optical properties||Optical clear view from all sides|
|Supplied in Fluidic slide?||No|
|Material chip||Borosilicate glass|
|Layer Thickness Carrier||0.4 mm (middle layer)|
|Material Carrier Layer||Borosilicate glass|
|Thickness Membrane||0.45µm pore size: 12µm thickness; 3µm µm pore size: 9µm; 8µm µm pore size: 16µm|
|Membrane Surface||~1 cm²|
|Membrane Pore Density||0.45µm pore size: 2.00 E+6 (cm2); 3µm µm pore size: 8.00 E+05 (cm2) 8µm pore size: 6.00 E+4 (cm2)|
|Membrane Surface Treatment||Cell culture treated|
Check and follow the Cleaning and sterilization procedure for resealable flow cells.
It is possible to couple Micronit OOC device to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of the oxygen in the culture chamber.
Micronit's OOC device is made of three glass layers. Glass has a very low gas permeability, making the system suitable for controlling gas concentrations. In case you use a syringe pump, the medium must be pre-conditioned to the right gas concentration. In case you use pneumatic pressurization systems, like Fluigent systems, specific gas mixtures can be used to condition the liquids during perfusion.
Have a look at this document about imaging systems.
When the bellow characteristics are overserved:
- Flow is periodically switching between the collection reservoir for the top and bottom flow path.
- Displacement of the membrane.
- Both flow paths are functioning correct, the reservoirs contains an amount of liquid that is expected based on the flow rates.
This is caused by droplet formation on the collection tubing. Each drop affects the flow a bit, resulting in changes in flow rates. This can be resolved by keeping the end of the tubing in the collection reservoir submerged in liquid.