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The Load 'n Seal design of the OOC chip holder asures tight connections to the organ-on-a-chip devices. Together with the Teflon Connection Kit PRO, 1mm ID, that contains the tubing and the perfluoroelastomer ferrules, you have the best solution for the organ-on-a-chip devices in the web store.
Features and Benefits:
Fast, easy and robust fluidics connections;
future proof thanks to replaceable inserts;
durable light-weight design;
large chip viewing area possible;
compatible with upright and inverted microscopes
The layer containing the cell culture membrane is placed in between the top and bottom OOC layers to form two separate flow chambers. This allows the flow of two different fluids, either liquids or gases, on either side of the membrane. This dynamic microfluidic flow approach enables new and innovative ways to culture cells and tissues while offering precise and continuous control during the complete culturing process.
In these two papers the Organ-on-a-chip platform was used:
Navarro-Tableros, Victor, et al. "Islet-Like Structures Generated In Vitro from Adult Human Liver Stem Cells Revert Hyperglycemia in Diabetic SCID Mice." Stem Cell Reviews and Reports (2018): 1-19.
|Product Code||Organ-on-a-Chip Platform|
|Dimensions||Chipholder: 128 mm x 85,4 mm x 20 mm|
|Sealing mechanism||Load ‘n Seal|
|Maximum operation temperature||80°C|
|Max. operating pressure||10 bar|
|Sealing material||Perlast (FFKM)|
|Contents||Connection Kit: Teflon tubing and 5 FFKM ferrules|
|Length||5 m tubing|
|Outer Diameter (OD)||1/16 Inch (~1.6 mm)|
|Inner Diameter (ID)||250 µm|
|Number of chips per pack||4 top and bottom layers, 12 membrane middle layers|
|Distance between channel and top surface||Thickness top layer 1.1 mm|
|Distance between channel and bottom surface||Thickness bottom layer 0.7 mm|
|Total chip thickness||2.6 mm (including middle layer)|
|Chip size||45 mm x15 mm|
|Channel width||11 mm, 2 channel, 1x on top of membrane, 1x on bottom of membrane|
|Channel height||~200 µm (per channel)|
|Number of Inlets||2 (1 inlet for flow channel on top membrane, 1 inlet for flow on bottom of membrane )|
|Number of outlets||2 (1 outlet for flow channel on top membrane, 1 outlet for flow on bottom of membrane )|
|Inlet/outlet hole sizes on top of the chip||1.7 mm|
|Inlet/outlet holes size at channel||0.75 mm|
|Optical properties||Optical clear view from all sides|
|Supplied in Fluidic slide?||No|
|Material chip||Borosilicate glass|
|Layer Thickness Carrier||0.4 mm (middle layer)|
|Material Carrier Layer||Borosilicate glass|
|Thickness Membrane||0.45µm pore size: 12µm thickness; 3µm µm pore size: 9µm; 8µm µm pore size: 16µm|
|Membrane Surface||~1 cm²|
|Membrane Pore Density||0.45µm pore size: 2.00 E+6 (cm2); 8µm pore size: 6.00 E+4 (cm2)|
|Membrane Surface Treatment||Cell culture treated|
Check and follow the Cleaning and sterilization procedure for resealable flow cells.
It is possible to couple Micronit OOC device to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of the oxygen in the culture chamber.
Micronit's OOC device is made of three glass layers. Glass has a very low gas permeability, making the system suitable for controlling gas concentrations. In case you use a syringe pump, the medium must be pre-conditioned to the right gas concentration. In case you use pneumatic pressurization systems, like Fluigent systems, specific gas mixtures can be used to condition the liquids during perfusion.
Have a look at this document about imaging systems.