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The Load 'n Seal design of the OOC chip holder asures tight connections to the organ-on-a-chip devices. Together with the Teflon Connection Kit PRO, 1mm ID, that contains the tubing and the perfluoroelastomer ferrules, you have the best solution for the organ-on-a-chip devices in the web store.
Features and Benefits:
Fast, easy and robust fluidics connections;
future proof thanks to replaceable inserts;
durable light-weight design;
large chip viewing area possible;
compatible with upright and inverted microscopes
The layer containing the cell culture membrane is placed in between the top and bottom OOC layers to form two separate flow chambers. This allows the flow of two different fluids, either liquids or gases, on either side of the membrane. This dynamic microfluidic flow approach enables new and innovative ways to culture cells and tissues while offering precise and continuous control during the complete culturing process.
|Product Code||Organ-on-a-Chip Platform|
|Dimensions||Chipholder: 128 mm x 85,4 mm x 20 mm|
|Sealing mechanism||Load ‘n Seal|
|Maximum operation temperature||80°C|
|Max. operating pressure||10 bar|
|Sealing material||Perlast (FFKM)|
|Inner Diameter (ID)||250 µm|
|Number of chips per pack||4 top and bottom layers, 12 membrane middle layers|
|Distance between channel and top surface||Thickness top layer 1.1 mm|
|Distance between channel and bottom surface||Thickness bottom layer 0.7 mm|
|Total chip thickness||2.6 mm (including middle layer)|
|Chip size||45 mm x15 mm|
|Channel width||11 mm, 2 channel, 1x on top of membrane, 1x on bottom of membrane|
|Channel height||~200 µm (per channel)|
|Number of Inlets||2 (1 inlet for flow channel on top membrane, 1 inlet for flow on bottom of membrane )|
|Number of outlets||2 (1 outlet for flow channel on top membrane, 1 outlet for flow on bottom of membrane )|
|Inlet/outlet hole sizes on top of the chip||1.7 mm|
|Inlet/outlet holes size at channel||0.75 mm|
|Optical properties||Optical clear view from all sides|
|Supplied in Fluidic slide?||No|
|Material chip||Borosilicate glass|
|Layer Thickness Carrier||0.4 mm (middle layer)|
|Material Carrier Layer||Borosilicate glass|
|Thickness Membrane||12 µm|
|Membrane Surface||~1 cm²|
|Membrane Pore Density||1.6 E6|
|Membrane Surface Treatment||Cell culture treated|
Check and follow the Cleaning and sterilization procedure for resealable flow cells.
It is possible to couple Micronit OOC device to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of the oxygen in the culture chamber.
Micronit's OOC device is made of three glass layers. Glass has a very low gas permeability, making the system suitable for controlling gas concentrations. In case you use a syringe pump, the medium must be pre-conditioned to the right gas concentration. In case you use pneumatic pressurization systems, like Fluigent systems, specific gas mixtures can be used to condition the liquids during perfusion.
Have a look at this document about imaging systems.
When the bellow characteristics are overserved:
- Flow is periodically switching between the collection reservoir for the top and bottom flow path.
- Displacement of the membrane.
- Both flow paths are functioning correct, the reservoirs contains an amount of liquid that is expected based on the flow rates.
This is caused by droplet formation on the collection tubing. Each drop affects the flow a bit, resulting in changes in flow rates. This can be resolved by keeping the end of the tubing in the collection reservoir submerged in liquid.