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Organ-on-a-Chip Platform

From: €3,437.00

To: €3,822.00

Price as configured: €0.00

Availability: In stock

This platform is an easy way to start your research.
We already assembled all the right components you need for cell culturing experiments.

The only thing left for you to do.. is choose how many chips you want and add this platform to your cart!

1 x Fluidic Connect PRO OOC (for organ-on-a-chip)   +€1,832.00
1 x 4-Pack Top and Bottom OOC Layers   +€672.00

Price as configured: €0.00

The Load 'n Seal design of the OOC chip holder asures tight connections to the organ-on-a-chip devices. Together with the Teflon Connection Kit PRO, 1mm ID, that contains the tubing and the perfluoroelastomer ferrules, you have the best solution for the organ-on-a-chip devices in the web store.

Features and Benefits:
Fast, easy and robust fluidics connections;
future proof thanks to replaceable inserts;
durable light-weight design;
large chip viewing area possible;
compatible with upright and inverted microscopes

The layer containing the cell culture membrane is placed in between the top and bottom OOC layers to form two separate flow chambers. This allows the flow of two different fluids, either liquids or gases, on either side of the membrane. This dynamic microfluidic flow approach enables new and innovative ways to culture cells and tissues while offering precise and continuous control during the complete culturing process.

Product CodeOrgan-on-a-Chip Platform
DimensionsChipholder: 128 mm x 85,4 mm x 20 mm
Sealing mechanismLoad ‘n Seal
Maximum operation temperature80°C
Max. operating pressure10 bar
Sealing materialPerlast (FFKM)
ContentNo
Inner Diameter (ID)250 µm
Number of chips per pack4 top and bottom layers, 12 membrane middle layers
Distance between channel and top surfaceThickness top layer 1.1 mm
Distance between channel and bottom surfaceThickness bottom layer 0.7 mm
Total chip thickness2.6 mm (including middle layer)
Chip size45 mm x15 mm
Channel width11 mm, 2 channel, 1x on top of membrane, 1x on bottom of membrane
Channel height~200 µm (per channel)
Number of Inlets2 (1 inlet for flow channel on top membrane, 1 inlet for flow on bottom of membrane )
Number of outlets2 (1 outlet for flow channel on top membrane, 1 outlet for flow on bottom of membrane )
Inlet/outlet hole sizes on top of the chip1.7 mm
Inlet/outlet holes size at channel0.75 mm
Optical propertiesOptical clear view from all sides
Supplied in Fluidic slide?No
Material chipBorosilicate glass
Layer Thickness Carrier0.4 mm (middle layer)
Material Carrier LayerBorosilicate glass
Membrane MaterialPET
Thickness Membrane12 µm
Membrane Surface~1 cm²
Membrane Pore Density1.6 E6
Membrane AppearanceTransparent
Membrane Surface TreatmentCell culture treated

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Quality

How do I clean and sterilize my resealable flow cells?

Is it possible to monitor oxygen levels?

It is possible to couple Micronit OOC device to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of the oxygen in the culture chamber.

Is it possible to control the oxygen concentration?

Micronit's OOC device is made of three glass layers. Glass has a very low gas permeability, making the system suitable for controlling gas concentrations. In case you use a syringe pump, the medium must be pre-conditioned to the right gas concentration. In case you use pneumatic pressurization systems, like Fluigent systems, specific gas mixtures can be used to condition the liquids during perfusion.

Is my OOC system compatible with my imaging systems?

The flow is periodically switching between top and bottom flow path.

When the bellow characteristics are overserved: 
- Flow is periodically switching between the collection reservoir for the top and bottom flow path. 
- Displacement of the membrane.
- Both flow paths are functioning correct, the reservoirs contains an amount of liquid that is expected based on the flow rates.

This is caused by droplet formation on the collection tubing. Each drop affects the flow a bit, resulting in changes in flow rates. This can be resolved by keeping the end of the tubing in the collection reservoir submerged in liquid.