Resealable flow cell - topconnect

In stock
As low as €550.00

per pack of 6

Pack of 6 resealable flow cell chips.

Resealable flow cells are an excellent choice when biological content needs to be added on-chip and can't be flushed inside. Resealable flow cells easily make a biological sensor. Just spot or dispense your biological content on the glass slide, place it inside the Fluidic Connect Pro chip holder for resealable flow cells, add the top layer and close the hinge.

Resealable flow cells consist of two layers; a top and bottom layer.

  • The bottom layer is a plain glass slide, with a thickness of 700µm.
  • The top layer is a 1100µm thick glass layer with an elastomer attached to it. The elastomer will define the flow path and seals the top layer.


More Information
Unit of measurementpack of 6
Interface typeTopconnect
Chip materialBorosilicate glass
Icon Label Description Type Size Download
PDF FLCRS_4515 Straight 5mm Drawing for the resealable flow cell with straight channel shape, 5mm width. PDF 112.4 KB Download
Customer Questions
Can this flow cell be customized?
Absolutely! Micronit is a key supplier for flow cells in the Next Generation Sequencing market and can manufacture flow cells competely according to your specific requirements.  What can be customized?

The channel shape. The channel height and width.
For wet etching its important to consider that channel height and minimal channel width are related.
{minimal channel width} = 2 * {etch depth} + {initial mask opening, typcial 5-10µm} 
Larger aspect ratios (relation between channel depth and width) are possible using dry etching. For this, a glass/silicon combination is most common. Choice of material. For instance, fused silica is a good option when UV light is used. Schott D263 bio can be a good alternative for some specific fluorescent labels.  Surface flatness. We can provide ultra flat surfaces.
How do I clean my resealable flow cell's / OOC top and bottom layers?
Cleaning Resealable flowcells are produced in cleanroom facilities and clean areas to prevent physical impurities. After use, cleaning might be required to remove organic residues. Please use distinguished cleaning processes for glass slides with and without a gasket. Glass slides with gasket: Can be cleaned with ethanol, isopropanol and acetone. The glass slides could be either immersed in the solvents or rinsed with them. Immersion might lead to diffusion of solvents into the gasket. Therefore, it is recommended to rinse the glass slide with the gasket thoroughly with water or buffer to remove residues. Only wipe with lint free tissue, otherwise the sealing might be affected negatively or completely lost. It is advised to not wipe the gasket as this might damage it and reduce its sealing abilities. Physical cleaning of the surface by either heat (with a maximum temperature of 140 °C) or oxygen plasma is possible. Cleaning with acids or bases has not been fully tested. Glass slides without gasket: Can be cleaned with organic solvents. Can be gently wiped with tissue. Physical cleaning of the surface by either heat up to 400 °C or oxygen plasma treatment is possible. Alkaline solution can be used to clean the surface, e.g. 1 M sodium hydroxide in water. Concentrated sulfuric acids could dissolve organic material, but are extremely corrosive, therefore it is not advised to use those. Hydrochloric acid could be used for cleaning, as well as bleach.
How do I sterilize my resealable flow cell's / OOC top and bottom layers?
Sterilization Resealable flowcells are not sterilized when delivered. Please use distinguished sterilization processes for glass slides with and without a gasket. Glass slides with gasket: Can be sterilized with ethanol. Dry heat of up to 140 °C can be applied. Can be autoclaved. Glass slides without gasket: Can be sterilized with ethanol. Can be autoclaved.
What is the oxygen permeability data for the elastomer used?
The oxygen permeability data from the exact material grade used is not available, however the data from a different grade of the same material family, can be found below.
What are the dimension of the available view window using the resealable / OOC chip holder?
The view window on the bottom of the resealable/OOC chip holder has the dimensions as found in the picture below:  
What is the roughness of the etched structures?
Wet etched structures are extremely smooth and have a roughness in Angstrom range. The structures are fully optical transparent. 
Large roughness for structures in glass chips is typical observed for structures manufactured by use of laser assisted manufacturing techniques or abrasion-based techniques like powder blasting. Almost all catalogue products from Micronit are manufactured using wet etching to create full transparent channels without substantial roughness. 
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