Resealable flow cell - topconnect

As low as €500.00
Availability:
In stock

Pack of 6 resealable flow cell chips.

Resealable flow cells are an excellent choice when biological content needs to be added on-chip and can't be flushed inside. Resealable flow cells easily make a biological sensor. Just spot or dispense your biological content on the glass slide, place it inside the Fluidic Connect Pro chip holder for resealable flow cells, add the top layer and close the hinge.

Resealable flow cells consist of two layers; a top and bottom layer.

  • The bottom layer is a plain glass slide, with a thickness of 700µm.
  • The top layer is a 1100µm thick glass layer with an elastomer attached to it. The elastomer will define the flow path and seals the top layer.

 

More Information
Unit of measurement6-Pack
Interface typeTopconnect
Chip materialBorosilicate glass
Icon Label Description Type Size Download
PDF FLCRS_4515 Straight 5mm PDF 112.4 KB Download
Customer Questions
Can this flow cell be customized?

Absolutely! Micronit is a key supplier for flow cells in the Next Generation Sequencing market and can manufacture flow cells competely according to your specific requirements. 

What can be customized?

  • The channel shape.
  • The channel height and width.
    For wet etching its important to consider that channel height and minimal channel width are related.
    {minimal channel width} = 2 * {etch depth} + {initial mask opening, typcial 5-10µm} 
    Larger aspect ratios (relation between channel depth and width) are possible using dry etching. For this, a glass/silicon combination is most common.
  • Choice of material. For instance, fused silica is a good option when UV light is used. Schott D263 bio can be a good alternative for some specific fluorescent labels. 
  • Surface flatness. We can provide ultra flat surfaces.

How do I clean my resealable flow cell's / OOC top and bottom layers?

Cleaning

Resealable flowcells are produced in cleanroom facilities and clean areas to prevent physical impurities. After use, cleaning might be required to remove organic residues. Please use distinguished cleaning processes for glass slides with and without a gasket.

Glass slides with gasket:

  • Can be cleaned with ethanol, isopropanol and acetone. The glass slides could be either immersed in the solvents or rinsed with them. Immersion might lead to diffusion of solvents into the gasket. Therefore, it is recommended to rinse the glass slide with the gasket thoroughly with water or buffer to remove residues.
  • Only wipe with lint free tissue, otherwise the sealing might be affected negatively or completely lost.
  • It is advised to not wipe the gasket as this might damage it and reduce its sealing abilities.
  • Physical cleaning of the surface by either heat (with a maximum temperature of 140 °C) or oxygen plasma is possible.
  • Cleaning with acids or bases has not been fully tested.

Glass slides without gasket:

  • Can be cleaned with organic solvents.
  • Can be gently wiped with tissue.
  • Physical cleaning of the surface by either heat up to 400 °C or oxygen plasma treatment is possible.
  • Alkaline solution can be used to clean the surface, e.g. 1 M sodium hydroxide in water.
  • Concentrated sulfuric acids could dissolve organic material, but are extremely corrosive, therefore it is not advised to use those. Hydrochloric acid could be used for cleaning, as well as bleach.

How do I sterilize my resealable flow cell's / OOC top and bottom layers?

Sterilization

Resealable flowcells are not sterilized when delivered. Please use distinguished sterilization processes for glass slides with and without a gasket.

Glass slides with gasket:

  • Can be sterilized with ethanol.
  • Dry heat of up to 140 °C can be applied.
  • Can be autoclaved.

Glass slides without gasket:

  • Can be sterilized with ethanol.
  • Can be autoclaved.

Would you like to submit a question? Please login first.
  • Worldwide delivery on all orders
  • Quotation first, print your quotation
  • Payments, pay safe and easily
Copyright © 2020-present Micronit B.V. All rights reserved.