T-shaped droplet generator - topconnect

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Pack of three T-shaped droplet generator chips.

Pack of 3 T-shaped droplet generator chips, for generating higly reproducible microsized droplets.

Microfluidic droplet generators are excellent tools for generating highly reproducible microsized droplets with much higher precision and repeatability compared to conventional methods.

By tuning the relative viscosities, surface tension, and velocities between the dispersed and the continuous phase, droplets of almost any size can be created.

Oil-in-Water (O/W) droplets can be generated directly using the glass chips. For Water-in-Oil droplets (W/O), choose the coated chips.

More Information
Unit of measurement3-Pack
Interface typeTopconnect
Chip materialBorosilicate glass
Supply format & dimensions

Supplied in a black polymer cardtridge with an external dimension of 75 x 25mm.
The functional glass element has a size of 45x15mm.

Chip thickness1800µm
Channel width100µm
Channel height20µm
Number of inlets2
Number of outlets1
Icon Label Description Type Size Download
pdf TSDG.2 - T-shaped droplet generator pdf 67.3 KB Download
pdf Surface wetting properties pdf 247.6 KB Download
pdf Flow rate instructions pdf 317.9 KB Download
pdf Using the right surfactants pdf 37.1 KB Download
pdf How to prevent clogging pdf 346 KB Download
Customer Questions
How do I clean my chips?

One simple but very effective way to clean a microchip is to flush an alkaline solution through the channels. A solution of 1 M sodium hydroxide in water works well, but a lower concentration might also be sufficient. If traces of the cleaning solution remain inside the chip after cleaning, rinse with water or ammonia. Note that caustic solutions can cause damage to e.g. the polyimide coating of fused silica capillaries. Further, plastic parts should not be exposed to alkaline solutions.

To remove particulate matter from your chip, a water bath with ultrasonic agitation can be used, preferably while flushing a watery solution through the channels using a Fluidic connection kit.

Glass microchips can be heated (e.g. >400° C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. Make sure you only heat the glass chip and not the plastic parts around it.

Concentrated sulfuric acid works well to dissolve organic material, such as fibres, that are difficult to remove with alkaline solutions. Always keep in mind that you are working with extremely corrosive material.

Please note that chips that were coated by Micronit have different guidelines for cleaning!

I need a pumping system for my setup. Which one do you recommend?

We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles.

To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers: https://www.fluigent.com/

 

Which flowrates should I use?

This depends on many things. For example on the type of fluid that you are using. Check our flowrate instructions to find out how to start.

I only see streaks of fluids but no droplets. How do I get the droplets?

Decrease your flowrate. Check our flowrate instructions for a more acurate explanation.

Which surfactants should I use?

Use our surfactant guide for advice on surfactants.

Should I use coated or uncoated droplet generators?

Have a look at our article about surface wetting properties.

Publication: Controlled and tunable polymer particles' production using a single microfluidic device

Amoyav, Benzion, and Ofra Benny "Controlled and tunable polymer particles’ production using a single microfluidic device." Applied Nanoscience (2018): 1-10. Abstract Microfluidics technology offers a new platform to control liquids under flow in small...

Publication: Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids

Lucio, Adam A., et al. "Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids." Scientific reports 7.1 (2017): 12022. Abstract Multicellular spheroids serve as an excellent platform to study tissue behavior...

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