Thin bottom flow cell - topconnect

In stock
As low as €214.00

per pack of 3

Pack of 3 thin bottom flow cell chips.

Application possibilities

A flow cell / observation cell is a sample cell designed so that liquids sample, for instance containing cell’s, proteins or particles, can be continuously flowed though a observation area.
Our glass flow’s cell are intended for situation where the optical properties are extremely critical and are suitable for high resolution imaging. They are often used in combination with fluorescent markers.

As alternative to continuously flowed sample it’s also possible to apply stopped flow observation by adding pressure control on both the cell inlet and outlet.   

Research examples can be for instance:

  • In-line sampling of liquids, mostly using a optical read out.
  • Cell observation / counting
  • Measurement of fluorescent response.  
  • Combined with surface chemistry they are used as base for NGS technology (Next-Generation Sequencing)

Unique selling point

  • The channels doesn’t have any substantial roughness and are fully optical transparent due to the etching process used.
  • Glass offers sublime optical transparency and low autofluorescence.
  • When fluorescent markers are used we have also a D263-Bio version available for even lower autofluorescence in some critical wave length areas.
  • Compatible with a #1 (03196, 03164) or #1.5 (03133) coverslip
  • Available in different channel dimensions.
  • Available with hydrophobic coating.

Key features of the products 

Item Coating Channel width Number of channels Channel height Thin bottom thickness Material
No coating, hyrophilic surface 500µm, 1500µm, 1000µm 50µm 175µm D263 Bio
03133-F Hyrophobic coated 500µm, 1500µm, 1000µm 3 50µm 175µm D263 Bio
03196 No coating, hyrophilic surface 500µm 3
(same channel size)
50µm 145µm D263 Eco
03196-F Hyrophobic coated 500µm 3
(same channel size)
50µm 145µm D263 Eco
03164 No coating, hyrophilic surface 1500µm 3
(same channel size)
50µm 145µm D263 Eco
03164-F Hyrophobic coated 1500µm 3
(same channel size)
50µm 145µm D263 Eco

D263 Bio offers a reduced autofluorescence when used in combination with fluorescent markers with emissions above 600nm.

This product is compatible with our Fluidic Connect Pro holder for chips supplied in a cartridge.
Because of the limited chip thickness it’s necessary to use the metal strips included with the holder, see here for more information.
To connect all three channels at the same time, you need six ferrules. The ferrules are available in sets of five. Make sure you order two sets if you want to connect three channels at the same time. 


More Information
Unit of measurementpack of 3
Interface typeTopconnect
Channel locationChannels are located 1050µm from the top surface. The distance form the bottom surface is 145µm (version with 3 different channels) and 175µm for the version with the same channels.
Chip thickness1.8mm
Chip materialBorosilicate glass
Number of inlets3
Number of outlets3
Icon Label Description Type Size Download
pdf FC_FLC50.3 Drawing pdf 70 KB Download
Customer Questions
Can this flow cell be customized?
Absolutely! Micronit is a key supplier for flow cells in the Next Generation Sequencing market and can manufacture flow cells competely according to your specific requirements.  What can be customized?

The channel shape. The channel height and width.
For wet etching its important to consider that channel height and minimal channel width are related.
{minimal channel width} = 2 * {etch depth} + {initial mask opening, typcial 5-10µm} 
Larger aspect ratios (relation between channel depth and width) are possible using dry etching. For this, a glass/silicon combination is most common. Choice of material. For instance, fused silica is a good option when UV light is used. Schott D263 bio can be a good alternative for some specific fluorescent labels.  Surface flatness. We can provide ultra flat surfaces.
When should I use the metal strips (spacers) as provided with the Fluidic Connect Pro holder?
When should I use the spacers? The metal strips are used for thin bottom chips and are also part of the holder for resealable flow cell's.
This are currently the following products (list might be incomplete): SKU Description Note 00746 Fluidic Connect Pro for resealable flow cells Normally already pre-assembled  00745 Insert set for resealable flow cells   03133 Thin bottom flow cell    03133-F Thin bottom flow cell - coated   03196 Thin bottom flow cell - 0.5mm   03196-F Thin bottom flow cell - 0.5mm - coated   03164 Thin bottom flow cell - 1.5mm   03164-F Thin bottom flow cell - 1.5mm - coated   FC_R50.332.3_PACK Serpentine channel / Microreactor, 20x50µm, 342mm length   FC_R50.332.3-F Serpentine channel / Microreactor, 20x50µm, 342mm length - Coated   03040 Serpentine channel / Microreactor, 45x140µm, 350mm length   ...
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