Droplet generation setup - topconnect (no pumps included)

From €2,075.00

To €7,698.00

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1 x Fluidic Connect Pro chip holder - topconnect - for chips in a cartridge   + €1,428.00
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This setup contains a chip holder, a connection kit, a selection of chips and other tools for a quick start.

This setup does not include pumps. For droplet generation, we recommend pressure based pumps to ensure an optimal experience. Pressure based pumps use stabilized pressure to push liquid out of a reservoir. Syringe pumps can generate a step wise movement in pressure release. This can result in pulsations, that cause instability in droplet generation. There are some high-end syringe pumps on the market that might overcome this issue, but we have limited experience with such pumps. For use in combination with high-end syringe pumps our Syringe to tube connection kit might be a useful addition. 

Droplet generation chips are available in the following types:

  • Focused Flow 
  • T-shaped
  • Parallel


Focused flow droplet generators are available in the following configurations:

SKU Nozzle size Coating Use for Recommended droplet size
(reference liquids)
00935 10µm Non - Hydrophilic Oil-in-water droplets 9µm< droplets <20µm
01341 Hydrophobic Water-in-oil droplets
02137 (Beta product) 20µm Non - Hydrophilic Oil-in-water droplets 15µm< droplets <30µm 
  Hydrophobic Water-in-oil droplets
00877 50µm Non - Hydrophilic Oil-in-water droplets 40µm< droplets <90µm
01366 Hydrophobic Water-in-oil droplets
00580 75µm Non - Hydrophilic Oil-in-water droplets 65µm< droplets <140µm 
00970 Hydrophobic Water-in-oil droplets

T-shaped droplet generators are available in the following configurations:

SKU Coating Use for Channel width Channel height
FC_TSDG.2_PACK Non - Hydrophilic Oil-in-water droplets 100µm 20µm
FC_TSDG.C.2_PACK Hydrophobic Water-in-oil droplets 100µm 20µm

Parallel droplet generators combine multiple focused flow droplet generators in parallel on a single chip with a shared inlet and outlet for increased production rates. They are available in the following configurations:

SKU Nozzle size Number of junctions Coating Use for Recommended droplet size
(reference liquids)
00923 75µm 8 lanes Non - Hydrophilic Oil-in-water droplets 65µm<droplets <140µm
02067 Hydrophobic Water-in-oil droplets



More Information
Unit of measurementBundle
ApplicationDroplet generation
Icon Label Description Type Size Download
pdf Flow rate instructions pdf 303.6 KB Download
pdf Surface wetting properties pdf 220.3 KB Download
pdf How to prevent clogging pdf 153 KB Download
pdf Using the right surfactants pdf 168.8 KB Download
pdf Droplet Generator Guide pdf 617 KB Download
pdf TSDG.2 - T-shaped droplet generator pdf 67.3 KB Download
PDF DGFF.P8.75 - Parallel droplet generator 75µm, 8 junctions -Drawing PDF 458.8 KB Download
PDF DGFF.50 - Drawing Drawing for 50µm Topconnect Focused Flow Droplet Generator. Items 01366 and 00877. PDF 179.6 KB Download
PDF DGFF.75 - Drawing Drawing for 75µm Topconnect Focused Flow Droplet Generator. Items 00970 and 00580. PDF 193.6 KB Download
pdf DGFF.10 - Drawing Drawing for 10µm Topconnect Focused Flow Droplet Generator. Items 01341 and 00935. pdf 159.2 KB Download
Customer Questions
How do I clean my chips?
One simple but very effective way to clean a microchip is to flush an alkaline solution through the channels. A solution of 1 M sodium hydroxide in water works well, but a lower concentration might also be sufficient. If traces of the cleaning solution remain inside the chip after cleaning, rinse with water or ammonia. Note that caustic solutions can cause damage to e.g. the polyimide coating of fused silica capillaries. Further, plastic parts should not be exposed to alkaline solutions. To remove particulate matter from your chip, a water bath with ultrasonic agitation can be used, preferably while flushing a watery solution through the channels using a Fluidic connection kit. Glass microchips can be heated (e.g. >400° C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. Make sure you only heat the glass chip and not the plastic parts around it. Concentrated sulfuric acid works well ...
I need a pumping system for my setup. Which one do you recommend?
We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles. To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers: https://www.fluigent.com/  
Which flowrates should I use?
This depends on many things. For example on the type of fluid that you are using. Check our flowrate instructions to find out how to start.
I only see streaks of fluids but no droplets. How do I get the droplets?
Decrease your flowrate. Check our flowrate instructions for a more acurate explanation.
Which surfactants should I use?
Use our surfactant guide for advice on surfactants.
How do I prevent clogging of my chips?
Have a look at our clogging prevention guide.
Should I use coated or uncoated droplet generators?
Have a look at our article about surface wetting properties.
Publication: Controlled and tunable polymer particles' production using a single microfluidic device
Amoyav, Benzion, and Ofra Benny "Controlled and tunable polymer particles’ production using a single microfluidic device." Applied Nanoscience (2018): 1-10. Abstract Microfluidics technology offers a new platform to control liquids under flow in small volumes. The advantage of using smallscale reactions for droplet generation along with the capacity to control the preparation parameters, making microfluidic chips an attractive technology for optimizing encapsulation formulations. However, one of the drawbacks in this methodology is the ability to obtain a wide range of droplet sizes, from sub-micron to microns using a single chip design. In fact, typically, droplet chips are used for micron-dimension particles, while nanoparticles’ synthesis requires complex chips design (i.e., microreactors and staggered herringbone micromixer). Here, we introduce the development of a highly...
Publication: Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids
Lucio, Adam A., et al. "Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids." Scientific reports 7.1 (2017): 12022. Abstract Multicellular spheroids serve as an excellent platform to study tissue behavior and tumor growth in a controlled, three-dimensional (3D) environment. While molecular and cellular studies have long used this platform to study cell behavior in 3D, only recently have studies using multicellular spheroids shown an important role for the mechanics of the microenvironment in a wide range of cellular processes, including during tumor progression. Despite the well-established relevance of mechanical cues to cell behavior and the numerous studies on mechanics using 2D cell culture systems, the spatial and temporal variations in endogenous cellular forces within growing multicellular aggregates remain unknown. Using cell-sized...
I have a Elveflow OB1 pressure based pump what connectors do I need?
Nuts and ferrules Elveflow uses 1/4-28 screw in connections on there reservoirs and sensors. To interface you will need Flangeless nuts 1/4-28 for 1/16" tubing, including compatible ferrules. Those nuts and ferrules are included in Elveflows "Kit Fittings Starter Pack Push-in".
This item wil look like this: In addition you will need our connection kit. Our Connection kit includes nuts or ferrules specificilly designed for our Fluidic Connect Pro, Fluidic Connect 4515 or side connect holders. Tubing included in "Kit Fittings Starter Pack Push-in" The 1/16"OD PTFE tubing included in the kit has a very large ID (1/32" ~0.79mm) and is from a relatively soft material, this is not so suitable for bot our Fluidic Connect Pro and Fluidic Connect 4515 holders as there's very limited wall thickness. We recommend alternative tubing, like ETFE with...
I have a Fluigent pressure based pump, what connectors do I need?
Lineup We recommend the dafault Fluigent kits (FLOW EZ™ SUPPLY KIT, FLUIWELL KIT or P-CAP KIT)
The P-cab or FLUIWELL KIT already contains 0.5m 1/16" OD FEP tubing with a ID of 500µm, when you use only limited pressures, you can try this tubing first and only order: For Fluidic Connect Pro our propriatary ferrules.  For Fluidic Connect 4515 our propriatary flatbottom nuts and ferrules.  For Sideconnect our propriatary ferrules.  For optimal performance we recommend our complete connection kit, the FEP tubing might...
What are the dimensions of the view window in the Fluidic Connect Pro for chips in a cartridge?
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