Organ-on-a-chip membrane layer

As low as €933.00
In stock

Pack of 12 middle layers, to be placed in between top and bottom organ-on-a-chip layers.

In organ-on-a-chip (OOC) processes, a cell culture membrane is placed in between two resealable glass slides to form two separate flow chambers. This allows two different flows, of either liquids or gases, on either side of the membrane. This dynamic microfluidic flow approach enables new and innovative ways to culture cells and tissues while offering precise and continuous control during the complete culturing process. The membrane layer together with a top and bottom OOC layer makes an organ-on-a-chip resealable flow cell.

The OOC middle layer is available in the following configurations:


Pore size

Membrane thickness
Pore density
[per cm2]
00738 0.45 12 2.00E+6
02081 1 11 2.00E+6
01206 3 9 8.00E+5
01060 8 16 6.00E+4


The pores are created using track-etching and have a random orientation:

Track etched pores (top view)


Using a small angle the random direction is shown:

More Information
Unit of measurement12-Pack
Interface typeTopconnect
Carrier thickness0.4mm
Carrier materialBorosilicate glass
Membrane materialPET
Membrane appearanceTransparent
Membrane surface treatmentCell culture treated
Icon Label Description Type Size Download
pdf Organ-on-a-chip drawing pdf 118.4 KB Download
pdf Organ-on-a-chip manual Manual that helps to get started with OOC using the resealable platform. pdf 1.5 MB Download
pdf Compatibility with cell imaging systems pdf 153.1 KB Download
Customer Questions
Is it possible to monitor oxygen levels?

It is possible to couple Micronit OOC devices to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of oxygen in the culture chamber.

Is my OOC system compatible with my imaging systems?

Have a look at this document about imaging systems.

The membrane is moving and flow is switching between top and bottom flow path. What is happening?

Always check that there’s no blockage in one of the flow paths, this can be verified by checking the amount of collected liquid in a specific time frame and compare it with the expected amount for the flow rate used.

If this isn't the case, droplet formation on the end of the tubing is the problem. Each falling droplet affects the flow a bit, resulting in changes in flow rates. Resolve this by keeping the end of the tubing in the collection reservoir submerged in liquid.

How do I clean my resealable flow cell's / OOC top and bottom layers?


Resealable flowcells are produced in cleanroom facilities and clean areas to prevent physical impurities. After use, cleaning might be required to remove organic residues. Please use distinguished cleaning processes for glass slides with and without a gasket.

Glass slides with gasket:

  • Can be cleaned with ethanol, isopropanol and acetone. The glass slides could be either immersed in the solvents or rinsed with them. Immersion might lead to diffusion of solvents into the gasket. Therefore, it is recommended to rinse the glass slide with the gasket thoroughly with water or buffer to remove residues.
  • Only wipe with lint free tissue, otherwise the sealing might be affected negatively or completely lost.
  • It is advised to not wipe the gasket as this might damage it and reduce its sealing abilities.
  • Physical cleaning of the surface by either heat (with a maximum temperature of 140 °C) or oxygen plasma is possible.
  • Cleaning with acids or bases has not been fully tested.

Glass slides without gasket:

  • Can be cleaned with organic solvents.
  • Can be gently wiped with tissue.
  • Physical cleaning of the surface by either heat up to 400 °C or oxygen plasma treatment is possible.
  • Alkaline solution can be used to clean the surface, e.g. 1 M sodium hydroxide in water.
  • Concentrated sulfuric acids could dissolve organic material, but are extremely corrosive, therefore it is not advised to use those. Hydrochloric acid could be used for cleaning, as well as bleach.

How do I sterilize my resealable flow cell's / OOC top and bottom layers?


Resealable flowcells are not sterilized when delivered. Please use distinguished sterilization processes for glass slides with and without a gasket.

Glass slides with gasket:

  • Can be sterilized with ethanol.
  • Dry heat of up to 140 °C can be applied.
  • Can be autoclaved.

Glass slides without gasket:

  • Can be sterilized with ethanol.
  • Can be autoclaved.

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