Help Center
Product group: EOR
What pressure and temperature can the EOR chip hold?
The EOR chip itself are manufactured from glass an can easily withstand up to 300C. However they are supplied in a black fluidic cartridge. This cartridge can width stand up to 80C.
There’s no sealant used, two layers of glass are fusion bonded together.
The chip can withstand easily up to 100 bar. However the external interfacing is more critical. Our Fluidic Connect holder offers the largest pressure, up to 80 bars (maybe less at elevated temperatures). Please note that the Fluidic Connect holder is a bit more complex in use. You need a bit of feeling to apply not too much force, which might brake the chip, but sufficient to obtain sufficient sealing. It might be necessary to tighten a bit more when pressure and temperature are increased. Please remove the pressure before additional tightening.
I'm using the Fluidic Connect 4515 holder (part of high pressure bundle), what alignment and fluidic port should I use?
The fluidic ports are on positions 3,8
Alignment can be done on positions 2,7 (or 4,9).
Product group: OOC
Is it possible to monitor oxygen levels?
It is possible to couple Micronit OOC devices to commercial optical readers for dissolved gases in the culture medium. This option allows for applications such as the monitoring of oxygen in the culture chamber.
Is my OOC system compatible with my imaging systems?
Have a look at this document about imaging systems.
The membrane is moving and flow is switching between top and bottom flow path. What is happening?
Always check that there’s no blockage in one of the flow paths, this can be verified by checking the amount of collected liquid in a specific time frame and compare it with the expected amount for the flow rate used.
If this isn't the case, droplet formation on the end of the tubing is the problem. Each falling droplet affects the flow a bit, resulting in changes in flow rates. Resolve this by keeping the end of the tubing in the collection reservoir submerged in liquid.
How do I clean my resealable flow cell's / OOC top and bottom layers?
Cleaning
Resealable flowcells are produced in cleanroom facilities and clean areas to prevent physical impurities. After use, cleaning might be required to remove organic residues. Please use distinguished cleaning processes for glass slides with and without a gasket.
Glass slides with gasket:
- Can be cleaned with ethanol, isopropanol and acetone. The glass slides could be either immersed in the solvents or rinsed with them. Immersion might lead to diffusion of solvents into the gasket. Therefore, it is recommended to rinse the glass slide with the gasket thoroughly with water or buffer to remove residues.
- Only wipe with lint free tissue, otherwise the sealing might be affected negatively or completely lost.
- It is advised to not wipe the gasket as this might damage it and reduce its sealing abilities.
- Physical cleaning of the surface by either heat (with a maximum temperature of 140 °C) or oxygen plasma is possible.
- Cleaning with acids or bases has not been fully tested.
Glass slides without gasket:
- Can be cleaned with organic solvents.
- Can be gently wiped with tissue.
- Physical cleaning of the surface by either heat up to 400 °C or oxygen plasma treatment is possible.
- Alkaline solution can be used to clean the surface, e.g. 1 M sodium hydroxide in water.
- Concentrated sulfuric acids could dissolve organic material, but are extremely corrosive, therefore it is not advised to use those. Hydrochloric acid could be used for cleaning, as well as bleach.
How do I sterilize my resealable flow cell's / OOC top and bottom layers?
Sterilization
Resealable flowcells are not sterilized when delivered. Please use distinguished sterilization processes for glass slides with and without a gasket.
Glass slides with gasket:
- Can be sterilized with ethanol.
- Dry heat of up to 140 °C can be applied.
- Can be autoclaved.
Glass slides without gasket:
- Can be sterilized with ethanol.
- Can be autoclaved.
What is the oxygen permeability data for the elastomer used?
The oxygen permeability data from the exact material grade used is not available, however the data from a different grade of the same material family, can be found below.
Product group: resealable
How do I clean my resealable flow cell's / OOC top and bottom layers?
Cleaning
Resealable flowcells are produced in cleanroom facilities and clean areas to prevent physical impurities. After use, cleaning might be required to remove organic residues. Please use distinguished cleaning processes for glass slides with and without a gasket.
Glass slides with gasket:
- Can be cleaned with ethanol, isopropanol and acetone. The glass slides could be either immersed in the solvents or rinsed with them. Immersion might lead to diffusion of solvents into the gasket. Therefore, it is recommended to rinse the glass slide with the gasket thoroughly with water or buffer to remove residues.
- Only wipe with lint free tissue, otherwise the sealing might be affected negatively or completely lost.
- It is advised to not wipe the gasket as this might damage it and reduce its sealing abilities.
- Physical cleaning of the surface by either heat (with a maximum temperature of 140 °C) or oxygen plasma is possible.
- Cleaning with acids or bases has not been fully tested.
Glass slides without gasket:
- Can be cleaned with organic solvents.
- Can be gently wiped with tissue.
- Physical cleaning of the surface by either heat up to 400 °C or oxygen plasma treatment is possible.
- Alkaline solution can be used to clean the surface, e.g. 1 M sodium hydroxide in water.
- Concentrated sulfuric acids could dissolve organic material, but are extremely corrosive, therefore it is not advised to use those. Hydrochloric acid could be used for cleaning, as well as bleach.
How do I sterilize my resealable flow cell's / OOC top and bottom layers?
Sterilization
Resealable flowcells are not sterilized when delivered. Please use distinguished sterilization processes for glass slides with and without a gasket.
Glass slides with gasket:
- Can be sterilized with ethanol.
- Dry heat of up to 140 °C can be applied.
- Can be autoclaved.
Glass slides without gasket:
- Can be sterilized with ethanol.
- Can be autoclaved.
What is the oxygen permeability data for the elastomer used?
The oxygen permeability data from the exact material grade used is not available, however the data from a different grade of the same material family, can be found below.