Droplet generation setup - sideconnect (no pumps included)
Combination of a Sideconnect chip holder (for 15x15 chips) and a set of optional tools and chips.
|Unit of measurement||Bundle|
|Flow rate instructions||303.6 KB||Download|
|How to prevent clogging||153 KB||Download|
|Using the right surfactants||168.8 KB||Download|
|Droplet Generator Guide||617 KB||Download|
|DGFF.SC.10 - Drawing||Drawing for 10µm sideconnect droplet generator. Items 01642 and 01365||297.8 KB||Download|
|DGFF.SC.75 - Drawing||Drawing for 75µm sideconnect droplet generator. Items 01641and 01368.||184.2 KB||Download|
|DGFF.SC.05 - Drawing||Drawing for 5µm sideconnect droplet generator. Items 01653 and 01364.||205.6 KB||Download|
|DGFF.SC.50 - Drawing||Drawing for 50µm sideconnect droplet generator. Items 01640 and 01367.||198.5 KB||Download|
|Sideconnect Manual||Manual for assembly of the Sideconnect holder with a chip and connection kit.||5.4 MB||Download|
I need a pumping system for my setup. Which one do you recommend?
We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles. To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers: https://www.fluigent.com/
Which flowrates should I use?
This depends on many things. For example on the type of fluid that you are using. Check our flowrate instructions to find out how to start.
I only see streaks of fluids but no droplets. How do I get the droplets?
Decrease your flowrate. Check our flowrate instructions for a more acurate explanation.
Which surfactants should I use?
Use our surfactant guide for advice on surfactants.
How do I prevent clogging of my chips?
Have a look at our clogging prevention guide.
Should I use coated or uncoated droplet generators?
Have a look at our article about surface wetting properties.
Publication: Controlled and tunable polymer particles' production using a single microfluidic device
Amoyav, Benzion, and Ofra Benny "Controlled and tunable polymer particles’ production using a single microfluidic device." Applied Nanoscience (2018): 1-10. Abstract Microfluidics technology offers a new platform to control liquids under flow in small volumes. The advantage of using smallscale reactions for droplet generation along with the capacity to control the preparation parameters, making microfluidic chips an attractive technology for optimizing encapsulation formulations. However, one of the drawbacks in this methodology is the ability to obtain a wide range of droplet sizes, from sub-micron to microns using a single chip design. In fact, typically, droplet chips are used for micron-dimension particles, while nanoparticles’ synthesis requires complex chips design (i.e., microreactors and staggered herringbone micromixer). Here, we introduce the development of a highly...
Publication: Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids
Lucio, Adam A., et al. "Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids." Scientific reports 7.1 (2017): 12022. Abstract Multicellular spheroids serve as an excellent platform to study tissue behavior and tumor growth in a controlled, three-dimensional (3D) environment. While molecular and cellular studies have long used this platform to study cell behavior in 3D, only recently have studies using multicellular spheroids shown an important role for the mechanics of the microenvironment in a wide range of cellular processes, including during tumor progression. Despite the well-established relevance of mechanical cues to cell behavior and the numerous studies on mechanics using 2D cell culture systems, the spatial and temporal variations in endogenous cellular forces within growing multicellular aggregates remain unknown. Using cell-sized oil ...
I have a Elveflow OB1 pressure based pump what connectors do I need?
Nuts and ferrules Elveflow uses 1/4-28 screw in connections on there reservoirs and sensors. To interface you will need Flangeless nuts 1/4-28 for 1/16" tubing, including compatible ferrules. Those nuts and ferrules are included in Elveflows "Kit Fittings Starter Pack Push-in".This item wil look like this: In addition you will need our connection kit. Our Connection kit includes nuts or ferrules specificilly designed for our Fluidic Connect Pro, Fluidic Connect 4515 or side connect holders. Tubing included in "Kit Fittings Starter Pack Push-in" The 1/16"OD PTFE tubing included in the kit has a very large ID (1/32" ~0.79mm) and is from a relatively soft material, this is not so suitable for bot our Fluidic Connect Pro and Fluidic Connect 4515 holders as there's very limited wall thickness. We recommend alternative tubing, like ETFE with a maximum of 0.5mm ID. This tubing is included in our <...
I have a Fluigent pressure based pump, what connectors do I need?
Lineup We recommend the dafault Fluigent kits (FLOW EZ™ SUPPLY KIT, FLUIWELL KIT or P-CAP KIT)The P-cab or FLUIWELL KIT already contains 0.5m 1/16" OD FEP tubing with a ID of 500µm, when you use only limited pressures, you can try this tubing first and only order: For Fluidic Connect Pro our propriatary ferrules. For Fluidic Connect 4515 our propriatary flatbottom nuts and ferrules. For Sideconnect our propriatary ferrules. For optimal performance we recommend our complete connection kit, the FEP tubing might be a bit soft limiting...