Focused flow droplet generator - sideconnect

SKU

FF_DROP_SIDE

Availability:

In stock

As low as €490.00

per pack of 3

Pack of 3 focused flow droplet generator chips for use in combination with our sideconnect interfacing. The chips are available with both a coated (hydrophobic) and uncoated (hydrophilic) channel surface.

Indicated reference droplet sizes are based on a reference experiment with Silicone oil 5cSt and DI water combined with 2% v/v Tween 20 or Tween 80.

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Details

Pack of 3 single nozzle side connect droplet generators with a nozzle etched on both sides, giving the following advantages:

Optimized nozzle geometry
Symmetric channels and nozzles for uniform droplet formation
Droplet production stable over a broader size and frequency range
Smaller droplet sizes available

This microfluidic droplet generator is an excellent tool for generating highly reproducible microsized droplets with much higher precision and repeatability compared to conventional methods.

By tuning the relative viscosities, surface tension, and velocities between the dispersed and the continuous phase, droplet size and frequency can be altered. Oil-in-Water (O/W) droplets can be generated directly using the glass chips. Also, these droplet generators are suitable for coating in order to form Water-in-Oil droplets (W/O).

Further specifics:

Droplet size can roughly be tuned from the size of the nozzle to be doubled
Suitable for foam, digital PCR, single cell analysis, emulsions etc.
Made from high quality glass, borosilicate, suitable for most biological and chemical applications

Application examples:

Cell, DNA, bead encapsulation for

drug discovery
drug studies
molecular biological studies
immunology studies
evolutional studies
enzyme catalysis studies

Food, paints, foams

Bubble formation
Mineral Oil Emulsion Production
Particle production - PLGA, PEGDA, gelatine, alginate, polystyrene, agaros
Drug delivery - creams, aerosols

Chemical

Droplet based micromixing
Droplet based microreactions

More Information

More Information
Unit of measurement	pack of 3
Application	Droplet generation
Interface type	Sideconnect
Chip material	Borosilicate glass
Chip thickness	1800µm
Channel location	Top and bottom
Number of inlets	2
Number of outlets	1

Documents

Label	Description	Type	Size	Download
DGFF.SC.10 - Drawing	Drawing for 10µm sideconnect droplet generator. Items 01642 and 01365	PDF	297.8 KB	Download
DGFF.SC.75 - Drawing	Drawing for 75µm sideconnect droplet generator. Items 01641and 01368.	PDF	184.2 KB	Download
DGFF.SC.50 - Drawing	Drawing for 50µm sideconnect droplet generator. Items 01640 and 01367.	PDF	198.5 KB	Download
DGFF.SC.05 - Drawing	Drawing for 5µm sideconnect droplet generator. Items 01653 and 01364.	PDF	205.6 KB	Download
Surface wetting properties		pdf	220.3 KB	Download
Flow rate instructions		pdf	303.6 KB	Download
How to prevent clogging		pdf	153 KB	Download
Using the right surfactants		pdf	168.8 KB	Download
Droplet Generator Guide		pdf	617 KB	Download

Product Questions (10)

Customer Questions

How do I clean my chips?

One simple but very effective way to clean a microchip is to flush an alkaline solution through the channels. A solution of 1 M sodium hydroxide in water works well, but a lower concentration might also be sufficient. If traces of the cleaning solution remain inside the chip after cleaning, rinse with water or ammonia. Note that caustic solutions can cause damage to e.g. the polyimide coating of fused silica capillaries. Further, plastic parts should not be exposed to alkaline solutions. To remove particulate matter from your chip, a water bath with ultrasonic agitation can be used, preferably while flushing a watery solution through the channels using a Fluidic connection kit. Glass microchips can be heated (e.g. >400° C) causing any organic material on the glass surface to degrade. Try to use lower temperatures first because burning the content could make it stick. Make sure you only heat the glass chip and not the plastic parts around it. Concentrated sulfuric acid works well ...

I need a pumping system for my setup. Which one do you recommend?

We recommend using a high precision pumping system. Regular syringe pumps often don't work very well for droplet generators. There are several high precision pumping systems on the market that work with different pumping principles. To name one, we'd like to mention that we have had positive experiences with the equipment Fluigent offers: https://www.fluigent.com/

Which flowrates should I use?

This depends on many things. For example on the type of fluid that you are using. Check our flowrate instructions to find out how to start.

I only see streaks of fluids but no droplets. How do I get the droplets?

Decrease your flowrate. Check our flowrate instructions for a more acurate explanation.

Which surfactants should I use?

Use our surfactant guide for advice on surfactants.

How do I prevent clogging of my chips?

Have a look at our clogging prevention guide.

Should I use coated or uncoated droplet generators?

Have a look at our article about surface wetting properties.

Publication: Controlled and tunable polymer particles' production using a single microfluidic device

Amoyav, Benzion, and Ofra Benny "Controlled and tunable polymer particles’ production using a single microfluidic device." Applied Nanoscience (2018): 1-10. Abstract Microfluidics technology offers a new platform to control liquids under flow in small volumes. The advantage of using smallscale reactions for droplet generation along with the capacity to control the preparation parameters, making microfluidic chips an attractive technology for optimizing encapsulation formulations. However, one of the drawbacks in this methodology is the ability to obtain a wide range of droplet sizes, from sub-micron to microns using a single chip design. In fact, typically, droplet chips are used for micron-dimension particles, while nanoparticles’ synthesis requires complex chips design (i.e., microreactors and staggered herringbone micromixer). Here, we introduce the development of a highly...

Publication: Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids

Lucio, Adam A., et al. "Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids." Scientific reports 7.1 (2017): 12022. Abstract Multicellular spheroids serve as an excellent platform to study tissue behavior and tumor growth in a controlled, three-dimensional (3D) environment. While molecular and cellular studies have long used this platform to study cell behavior in 3D, only recently have studies using multicellular spheroids shown an important role for the mechanics of the microenvironment in a wide range of cellular processes, including during tumor progression. Despite the well-established relevance of mechanical cues to cell behavior and the numerous studies on mechanics using 2D cell culture systems, the spatial and temporal variations in endogenous cellular forces within growing multicellular aggregates remain unknown. Using cell-sized...

What's important in selection of a suitable microscope objective (regular bottom thickness chips)?

A inverted miscope is recommend, as most surface for observation is on the bottom (non-inlet side).
The objective working distance is a critical parameter for selection of an objective. Most default objectives are indented for a #1.5 cover slip which is only 170µm thick, where the thickness below channel is mostly in the range of 400-900µm.
Those objectives with longer working distance are often called non-coverglass objectives.
Where possible we would recommend to work dry. In most cases it should be possible to use the channel edge or other well defined point as reference for manual size corrections, this would reduce the need for corrections by the objective.

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